FIP IN-PERSON Seminar "Light-sheet microscopy for cell biology"

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Wed, 04/06/2022 - 12:00 to 13:00

Dr. Bo Huang


Dr. Bo Huang, Professor, Department of Pharmaceutical Chemistry, Department Biochemistry and Biophysics (joint), University of California, San Francisco

Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To visualize their spatial localization, temporal dynamics and activity profiles, fluorescence microscopy has been an indispensable tool. The challenge, though, is the trade-off of spatial resolution, temporal resolution and observation duration and phototoxicity. Light-sheet microscopy has recently emerged as a solution to address the latter three factors, though the typical two-objective configuration still limits high-resolution subcellular imaging, both because of the limited numerical aperture and geometric constraints on sample formats. Towards an “ideal” microscope for live cell imaging, we have developed both the dual-objective “open-top” configuration and the single-objective eSPIM system, enabling large scale monitoring of cellular protein dynamics. We have also identified the best “shape” of the light sheet through unbiased computational optimization.
Dr. Bo Huang is a Professor of Pharmaceutical Chemistry and of Biochemistry and Biophysics (joint) at University of California, San Francisco, and a Chan Zuckerberg Biohub Investigator. He received his B.S. degree in Chemistry from Peking University, China, in 2001 and Ph. D. degree in Chemistry at Stanford University in 2006. After finishing postdoc work at Harvard University in 2009, he joined UCSF as an Assistant Professor. He was promoted to Associate Professor in 2014 and Professor in 2017. Dr. Huang’s research work encompasses the areas of optical microscopy, probe and cell engineering, protein biophysics and cell biology. He has received many awards including the GE Healthcare and Science Prize for Young Life Scientists, Searle Scholarship, Packard Fellowship for Science and Engineering, the NIH Director’s New Innovator Award, the American Society for Cell Biology Young Life Scientist Award, and UCSF Byers Award for Basic Science.