Chemistry Seminar Presented by Lu Wei: Functional Bond-Selective Microscopy for Subcellular Biology: From Quantitative Imaging and Sensing to Single-Molecule Analysis

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Tue, 04/23/2024 - 11:45 to 12:45

Presenter

Lu Wei, Cal Tech

The Department of Chemistry is excited to host Lu Wei (Cal Tech). To learn more about the Wei lab's work, please visit their research page:https://www.weilab.caltech.edu/ Functional Bond-Selective Microscopy for Subcellular Biology: From Quantitative Imaging and Sensing to Single-Molecule Analysis Advances in optical spectroscopy and microscopy have revolutionized our understanding in live biological functions at the sub-cellular levels. In this seminar, I will present our recent efforts in developing and applying next-generation bond-selective spectro-microscopy for retrieving functional chemical information in live cells and neurons. I will first discuss the coupling of the stimulated Raman scattering (SRS) imaging, a nonlinear Raman imaging modality, with newly developed biorthogonal chemical strategies for quantitative subcellular analysis of protein aggregates in neurodegenerative diseases and for intracellular local environmental sensing through alkyne- hydrogen-deuterium exchange (Alkyne-HDX). I will then present a new mid-infrared near-infrared double-resonance imaging technique, BonFIRE, for bond-selective fluorescence imaging with single-molecule sensitivity. This microscopy platform should allow new capabilities for wide-field super-multiplex imaging and vibrational life-time imaging for sensing heterogeneous cellular interactions and environment. Hosted by Kevin Welsher