Alberto Bartesaghi


Associate Professor of Computer Science

Dr. Bartesaghi is an Associate Professor in the departments of Computer Science, Biochemistry and Electrical and Computer Engineering at Duke University. The Bartesaghi Lab focuses on the development of machine learning approaches to determine the structure of macromolecular complexes of general biomedical interest using single-particle cryo-electron microscopy, cryo-electron tomography, and sub-volume averaging. Some of our targets include glycoproteins of enveloped viruses like HIV, Influenza and Ebola, transporters and channels involved in signaling and metabolism, GPCRs, DNA-targeting CRISPR-Cas surveillance complexes, and targets for cancer drugs. The lab also works more broadly in the fields of deep learning and artificial intelligence, computer vision, biomedical imaging, and high-performance computing.

Appointments and Affiliations

  • Associate Professor of Computer Science
  • Associate Professor of Biochemistry
  • Associate Professor in the Department of Electrical and Computer Engineering
  • Faculty Network Member of the Duke Institute for Brain Sciences

Contact Information

  • Office Location: 308 Research Drive, LSRC D338, Durham, NC 27708-0129
  • Office Phone: +1 919 660 6579
  • Email Address:
  • Websites:


  • D.Phil. University of Minnesota, Twin Cities, 2005

Research Interests

Image analysis, data science, computer vision, machine learning, image reconstruction, cryo-electron microscopy, protein structure

Courses Taught

  • COMPSCI 562: High-Resolution Cryo-Electron Microscopy Image Analysis
  • COMPSCI 393: Research Independent Study
  • COMPSCI 362: Introduction to Computational Imaging
  • COMPSCI 290: Topics in Computer Science
  • CBB 562: High-Resolution Cryo-Electron Microscopy Image Analysis
  • BIOCHEM 700: Graduate Training Internship
  • BIOCHEM 593: Research Independent Study
  • BIOCHEM 562: High-Resolution Cryo-Electron Microscopy Image Analysis
  • BIOCHEM 393: Research Independent Study

In the News

Representative Publications

  • Bouvette, J; Liu, H-F; Du, X; Zhou, Y; Sikkema, AP; da Fonseca Rezende E Mello, J; Klemm, BP; Huang, R; Schaaper, RM; Borgnia, MJ; Bartesaghi, A, Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography., Nat Commun, vol 12 no. 1 (2021) [10.1038/s41467-021-22251-8] [abs].
  • Kang, Y; Kuybeda, O; de Waal, PW; Mukherjee, S; Van Eps, N; Dutka, P; Zhou, XE; Bartesaghi, A; Erramilli, S; Morizumi, T; Gu, X; Yin, Y; Liu, P; Jiang, Y; Meng, X; Zhao, G; Melcher, K; Ernst, OP; Kossiakoff, AA; Subramaniam, S; Xu, HE, Cryo-EM structure of human rhodopsin bound to an inhibitory G protein., Nature, vol 558 no. 7711 (2018), pp. 553-558 [10.1038/s41586-018-0215-y] [abs].
  • Bartesaghi, A; Aguerrebere, C; Falconieri, V; Banerjee, S; Earl, LA; Zhu, X; Grigorieff, N; Milne, JLS; Sapiro, G; Wu, X; Subramaniam, S, Atomic Resolution Cryo-EM Structure of β-Galactosidase., Structure (London, England : 1993), vol 26 no. 6 (2018), pp. 848-856.e3 [10.1016/j.str.2018.04.004] [abs].
  • Guo, TW; Bartesaghi, A; Yang, H; Falconieri, V; Rao, P; Merk, A; Eng, ET; Raczkowski, AM; Fox, T; Earl, LA; Patel, DJ; Subramaniam, S, Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex., Cell, vol 171 no. 2 (2017), pp. 414-426.e12 [10.1016/j.cell.2017.09.006] [abs].
  • Aguerrebere, C; Delbracio, M; Bartesaghi, A; Sapiro, G, Fundamental limits in multi-image alignment, IEEE Transactions on Signal Processing, vol 64 no. 21 (2016), pp. 5707-5722 [10.1109/TSP.2016.2600517] [abs].
  • Merk, A; Bartesaghi, A; Banerjee, S; Falconieri, V; Rao, P; Davis, MI; Pragani, R; Boxer, MB; Earl, LA; Milne, JLS; Subramaniam, S, Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery., Cell, vol 165 no. 7 (2016), pp. 1698-1707 [10.1016/j.cell.2016.05.040] [abs].